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目的:研究微小RNA-203(miR-203)靶向ΔNp63对口腔鳞状细胞癌(OSCC)细胞增殖、侵袭、凋亡和干细胞特性的影响.方法:TCGA数据库分析miR-203和ΔNp63在头颈鳞状细胞癌及其邻近正常组织中的表达.采用RT-qPCR法检测OSCC细胞系(Tca8113、CAL-27、SCC-9)和正常人口腔角质形成细胞(NHOK)中miR-203和ΔNp63 mRNA表达.将miR-NC、miR-203 mimic、pcDNA、pcDNA-ΔNp63分别转染及miR-203 mimic和pcDNA-ΔNp63共转染CAL-27细胞.Western blotting法检测ΔNp63、Bax、Bcl-2、Ki67、MMP-2、E-cadherin、N-cadherin、Lin28B、SOX2和OCT4蛋白表达.荧光素酶报告实验分析miR-203和ΔNp63的靶向关系.CCK8、Transwell、流式细胞术分别检测细胞活力、侵袭能力、凋亡和ALDH阳性细胞数目.构建裸鼠移植瘤模型,检测miR-203对体内肿瘤的作用.结果:miR-203在头颈鳞状细胞癌组织和OSCC细胞中表达下调,ΔNp63表达上调(P<0.05).miR-203靶向负调控ΔNp63的表达.miR-203组ΔNp63、Ki67、MMP-2、N-cadherin、Bcl-2、Lin28B、SOX2和OCT4表达及CAL-27细胞活力降低,侵袭细胞数和ALDH阳性细胞数明显减少,细胞凋亡率及Bax和E-cadherin表达升高(均P<0.05).pcDNA-ΔNp63组结果与之相反,且过表达miR-203可逆转ΔNp63对CAL-27细胞活力、侵袭和干细胞样特性的促进作用(P<0.05).miR-203能抑制口腔鳞癌细胞CAL-27裸鼠移植瘤的生长,并下调肿瘤组织ΔNp63表达.结论:miR-203通过抑制ΔNp63表达在OSCC细胞中发挥抑癌作用.“,”Objective:To investigate the effect of miR-203 tar-geting ΔNp63 on proliferation, invasion, apoptosis and stem-cell characteristics of oral squamous cell carcinoma (OSCC) cells. Methods: TCGA database was used to analyze the ex-pression of miR-203 and ΔNp63 in head and neck squamous cell carcinoma and adjacent normal tissues. RT-qPCR was used to detect the expression of miR-203 and ΔNp63 mRNA in OSCC cells (Tca8113, CAL-27, and SCC-9) and normal hu-man oral keratinocytes (NHOK). The CAL-27 cells were trans-fected with miR-NC, miR-203 mimic, pcDNA, or pcDNA- ΔNp63, and co-transfected with miR-203 mimic and pcDNA- ΔNp63. The expressions of ΔNp63, Bax, Bcl-2, Ki67, MMP-2, E-cadherin, N-cadherin, Lin28B, SOX2 and OCT4 were detect-ed by Western blotting. Luciferase reporter assay was used to analyze the targeting relationship between miR-203 and ΔNp63. CCK8, Transwell, and flow cytometry were performed respec-tively to detect cell viability, invasion, apoptosis and the num-ber of ALDH positive cells. A nude mouse xenograft tumor was constructed to detect the effect of miR-203 on tumors in vi-vo. Results: The expression of miR-203 was down-regulated while the expression of ΔNp63 was up-regulated in head and neck squamous cell carcinoma and OSCC cells (P<0.05). miR-203 targeted and negatively regulated the expression of ΔNp63. The expressions of ΔNp63, Ki67, MMP-2, N-cad-herin, Bcl-2, Lin28B, SOX2 and OCT4, the viability of CAL-27 cells, the number of invasive cells and ALDH-positive cells were decreased significantly, while the apoptosis rate and the protein expression levels of Bax and E-cadherin were increased in miR-203 group (P<0.01). The results of ΔNp63 group were opposite. miR-203 mimic reversed the promotive effects of overexpressed ΔNp63 on CAL-27 cell viability, invasion and stem cell-like properties (P<0.05). miR-203 inhibited tumor growth in nude mice translanted with OSCC cell line and downregulated ΔNp63 expression. Conclusion:miR-203 plays a tumor-suppressive role in OSCC by inhibiting the expression of ΔNp63.