The positive ecological interaction between gap formation and natural regeneration has been examined but little research has been carried out on the effects of gaps on natural regeneration in forests under different intensities of disturbance. This study evaluates the composition, diversity, regeneration density and abundance of natural regeneration of tree species in gaps in undisturbed, intermittently dis-turbed, and disturbed forest sites. Bia Tano Forest Reserve in Ghana was the study area and three gaps each were selected in the three forest site categories. Ten circular subsampling areas of 1 m 2 were delineated at 2 m spacing along north, south, east, and west transects within individual gaps. Data on natural regeneration < 350 cm height were gathered. The results show that the intensity of disturbance was dispro-portional to gap size. Species diversity differed significantly between undisturbed and disturbed sites and, also between intermittently disturbed and disturbed sites for Simpson\'s (1-D), Equitability (J), and Berger–Parker (B–P) indices. However, there was no significant difference among for-est sites for Shannon diversity (H) and Margalef richness (MI) indices. Tree species composition on the sites differed. Regeneration density on the disturbed site was significantly higher than on the two other sites. Greater abundance and density of shade-dependent species on all sites identified them as opportunistic replacements of gap-dependent pio-neers. Pioneer species giving way to shade tolerant species is a natural process, thus make them worst variant in gap regeneration.