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目的:建立胃和冲剂Ⅰ号的质量标准。方法:采用薄层色谱法(TLC)对胃和冲剂Ⅰ号中的半夏、黄芪、白芍、党参进行鉴别;采用高效液相色谱法(HPLC)测定制剂中芍药苷含量。结果:薄层色谱斑点清晰,分离良好,阴性对照无干扰。芍药苷在(0.05848~0.6433)μg之间呈良好的线性关系。平均加样回收率为103.56%(n=6),RSD=0.70%。结论:该方法灵敏、简便、准确和重复性好,可作为胃和冲剂Ⅰ号的质量控制标准。
Objective: To establish the quality standard for Weihei I. METHODS: Thin-layer chromatography (TLC) was used to identify Pinellia ternate, Radix Astragali, Radix paeoniae Radix and Codonopsis Pilosula, and HPLC was used to determine the content of paeoniflorin in the preparation. RESULTS: Thin-layer chromatography showed clear spots and good separation. Negative controls showed no interference. Paeoniflorin showed a good linear relationship between (0.05848-0.6433) μg. The average addition recovery was 103.56% (n=6) and RSD=0.70%. Conclusion: This method is sensitive, simple, accurate and reproducible. It can be used as a quality control standard for Weihe Granule I.