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目的研究五环三萜类柴胡皂苷单体Bp3对解脲脲原体四环素耐药株的作用及其与四环素的相互作用。方法参照美国临床实验室标准化协会标准,采用棋盘微量稀释法,测定单用Bp3、四环素及两者联合使用时对20株四环素耐药解脲脲原体的最低抑菌浓度(MIC),计算部分抑菌浓度(FIC)指数,判定两药相互作用。结果单独用药时Bp3及四环素对解脲脲原体四环素耐药株MIC的几何均数值(GM值)分别为1.234 mg.L-1和0.948 mg.L-1,联合用药时Bp3和四环素的GM值分别降低为0.692 mg.L-1和0.271 mg.L-1。2种药物单用和联合使用时MIC值的差异均有统计学意义(P<0.05),联用时在20株耐药株均表现为协同或相加作用。结论五环三萜类柴胡皂苷单体Bp3对解脲脲原体四环素耐药株有抑制作用,且与四环素有协同或相加作用。
Objective To study the effect of pentacyclic triterpene saikosaponin Bp3 on tetracycline-resistant strains of Ureaplasma urealyticum and its interaction with tetracycline. Methods According to the standards of American Society of Clinical Laboratory Standards, the minimum inhibitory concentration (MIC) of 20 strains of tetracycline-resistant Ureaplasma urealyticum was determined by chessboard microdilution when using Bp3, tetracycline and their combination alone. Bacteriostatic concentration (FIC) index, to determine the interaction between the two drugs. Results The geometric mean values (GM) of Bp3 and tetracycline against MIC of tetracycline strains of Ureaplasma urealyticum were 1.234 mg.L-1 and 0.948 mg.L-1, respectively. The combination of Bp3 and tetracycline GM Values were reduced to 0.692 mg.L-1 and 0.271 mg.L-1.2 kinds of drugs alone and in combination MIC values were statistically significant differences (P <0.05), combined with 20 resistant strains All showed synergistic or additive effects. Conclusions The pentacyclic triterpene saikosaponin Bp3 can inhibit the tetracycline-resistant strains of Ureaplasma urealyticum and has synergistic effect with tetracycline.