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目的:建立同时测定当归养血丸中芍药苷、白术内酯Ⅰ和白术内酯Ⅲ含量的方法。方法:采用HPLC法,ZORBAX-C18柱(4.6mm×250mm,5μm),流动相为乙腈(A)∶0.2%磷酸溶液(B),进行梯度洗脱(0-40min,A18%-62%,B82%-38%),流速为0.8ml·min~(-1),柱温:30℃,检测波长为222nm。结果:芍药苷、白术内酯Ⅰ和白术内酯Ⅲ分别在(0.014～0.276)mg·ml~(-1)(r=0.9996),(0.6～12.0)μg·ml~(-1)(r=0.9995)和(1.3～26.0)μg·ml~(-1)(r=0.9998)浓度范围内均有良好的线性关系,平均加样回收率分别为99.0%,98.0%,97.9%;RSD均小于2.0%。结论:本方法简便,准确,重复性好,可用于该制剂的质量控制。
Objective: To establish a method for the simultaneous determination of paeoniflorin, atractylenolide A and atractylenolide Ⅲ in Danggui Yangxue Wan. METHODS: The mobile phase consisted of acetonitrile (A): 0.2% phosphoric acid (B) and gradient elution (0-40 min, A18% -62%, HPLC) on a ZORBAX-C18 column (4.6 mm × 250 mm, B82% -38%). The flow rate was 0.8ml · min -1. The column temperature was 30 ℃ and the detection wavelength was 222nm. Results: Paeoniflorin, atractylenolide Ⅰ and atractylenolide Ⅲ were stable in the range of (0.014 ~ 0.276) mg · ml -1 (r = 0.9996), (0.6 ~ 12.0) μg · ml -1 = 0.9995) and (1.3 ~ 26.0) μg · ml ~ (-1) (r = 0.9998), respectively. The average recoveries were 99.0%, 98.0% and 97.9% Less than 2.0%. Conclusion: The method is simple, accurate, reproducible and can be used for the quality control of the preparation.