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为了进一步理解光在夏枯草生长和有效成分积累中的作用,本研究以LED灯管为光源,分别设置4组不同强度的白光和红光,探究光强和光质对夏枯草幼苗生长指标、光合特性指标、迷迭香酸含量和迷迭香酸生源途径关键酶基因表达的影响。结果发现提高两种光质的强度均可促进夏枯草幼苗的生长,且在同等强度条件下,红光较白光有更加明显的促进作用。迷迭香酸的含量随着光照强度的提高呈上升趋势,同等强度的两种光质相比,红光在较低强度条件下抑制迷迭香酸的积累,较高强度条件下两种光质的调控作用则无明显差异。迷迭香酸生源途径上8个关键酶基因表达量的测定结果表明,PAL、C4H、TAT和RAS的表达受两种光质的诱导,与迷迭香酸含量具有较好的一致性。本研究的结果表明,适当强度的红光可以在不影响夏枯草迷迭香酸积累的情况下促进其生长,在高产优质的夏枯草药材生产中具有较好的应用前景。
In order to further understand the role of light in the accumulation of Prunella vulgaris and its active ingredients, this study used LED lamp as light source, and set up 4 groups of white and red light with different intensities respectively to investigate the effects of light intensity and light quality on Prunella vulgaris seedling growth index, photosynthesis Traits, rosmarinic acid content and the expression of key enzyme genes in the pathway of rosmarinic acid. The results showed that increasing the light intensity could promote the growth of Prunella vulgaris seedlings, and under the same intensity conditions, red light had a more obvious promotion effect than white light. The content of rosmarinic acid increased with the increase of light intensity. Compared with the two light qualities of the same intensity, red light inhibited the accumulation of rosmarinic acid at lower intensity. Under the condition of higher intensity, There is no obvious difference in the regulation of quality. The results showed that PAL, C4H, TAT and RAS were induced by two light components, which was in good agreement with rosmarinic acid content. The results of this study showed that the red light of appropriate intensity can promote the growth of the prunella rosea acid without affecting the accumulation of the prunella rosea and has a good application prospect in the production of high yield and quality prunella medicinal materials.