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AIM TO probe into the genetic susceptibility of HLA-DRB1alleles to esophageal carcinoma in Han Chinese in HubeiProvince.METHODS:HLA-DRB1 allele polymorphisms were typedby polymerase chain reaction with sequence-specific primers(PCR-SSP)in 42 unrelated patients with esophageal cancerand 136 unrelated normal control subjects and the associatedHLA-DRB1 allele was measured by nucleotide sequenceanalysis with PCR.SAS software was used in statistics.RESULTS:Allele frequency(AF)of HLA-DRB1~*0901 wassignificantly higher in esophageal carcinoma patients thanthat in the normal controls(0.2500 vs0.1397,P=0.028,theodds ratio 2.053,etiologic fraction 0.1282).After analyzedthe allele nucleotide sequence of HLA-DRB1~*0901 whichapproachs to the corresponded exon 2 sequence of the allelein genebank.There was no association between patientsand controls in the rested HLA-DRB1 alleles.CONCLUSION:HLA-DRB1~*0901 allele is more common inthe patients with esophageal carcinoma than in the healthycontrols,which is positively associated with the patients ofHubei Han Chinese.Individuals carrying HLA-DRB1~*0901may be susceptible to esophageal carcinoma.
AIM TO probe into the genetic susceptibility of HLA-DRB1alleles to esophageal carcinoma in Han Chinese in Hubei Province. METHODS: HLA-DRB1 allele polymorphisms were typed by polymerase chain reaction with sequence-specific primers (PCR-SSP) in 42 unrelated patients with esophageal cancerand 136 unrelated normal control subjects and the associated HLA-DRB1 allele was measured by nucleotide sequence analysis with PCR.SAS software was used in statistics .RESULTS: Allele frequency (AF) of HLA-DRB1 ~ * 0901 wassignificantly higher in esophageal carcinoma patients thanthat in the normal controls (0.2500 vs 0.1397, P = 0.028, theodds ratio 2.053, etiologic fraction 0.1282) .After analyzed the allele nucleotide sequence of HLA-DRB1 ~ * 0901 whichapproachs to the corresponded exon 2 sequence of the allelein genebank. There was no association between patients and controls in the rested HLA-DRB1 alleles. CONCLUSION: HLA-DRB1 ~ * 0901 allele is more common inthe patients with esophageal carcinoma than in the healthy controls, which is positively associated with the patients ofHubei Han Chinese.Individuals carrying HLA-DRB1 ~ * 0901may be susceptible to esophageal carcinoma.