论文部分内容阅读
目的探讨日本血吸虫可溶性虫卵抗原(SEA)对ApoE-/-小鼠动脉粥样硬化的保护作用及其免疫调节机制。方法设置实验组1和2,实验组1小鼠从第1周开始高脂饮食喂养,分为预防组和对照组,第1周时分别腹腔注射SEA及PBS,每周1次,共4次;实验组2小鼠高脂饮食喂养至第14周,分为治疗组和对照组,第14周时分别注射SEA及PBS,每周1次,共4次。第22周处死小鼠,观察SEA对动脉粥样硬化的干预作用以及小鼠体内的细胞因子和调节性T细胞亚群CD4+CD25+FoxP3+T细胞水平的变化。结果给予SEA后,与相应对照组比较,预防组和治疗组小鼠血清总胆固醇含量均显著降低,外周血TNF-α、IL-10水平均显著下降;预防组小鼠动脉粥样硬化斑块面积被显著抑制,但治疗组无明显变化。第22周时,预防组外周血CD4+CD25+FoxP3+T细胞占CD4+T细胞比例为(4.4±0.9)%,与对照组[(2.6±0.3)%]相比,差异有统计学意义(P<0.05);但治疗组细胞比例给予SEA前后差异无统计学意义(P<0.05)。结论SEA能降低ApoE-/-小鼠血清总胆固醇水平;预防性给予SEA具有抑制动脉粥样硬化作用,其主要机制是在疾病初始阶段抑制炎症因子产生Treg细胞增殖。
Objective To investigate the protective effect of Schistosoma japonicum soluble egg antigen (AECA) on atherosclerosis in ApoE - / - mice and its immunoregulatory mechanism. Methods The experimental groups 1 and 2 were set up. The experimental group 1 mice were fed with high-fat diet from the first week and divided into prevention group and control group. The first week, the mice were injected intraperitoneally with SEA and PBS once a week for 4 times The experimental group 2 mice were fed with high-fat diet until the 14th week, and divided into treatment group and control group. SEA and PBS were injected at the 14th week once a week for 4 times. Mice were sacrificed on the 22nd week to observe the intervention effect of SEA on atherosclerosis and the changes of the level of CD4 + CD25 + FoxP3 + T cells in mice. Results Compared with the corresponding control group, the levels of serum total cholesterol and the levels of TNF-α and IL-10 in peripheral blood of the prophylaxis and treatment groups were significantly decreased after the administration of SEA. The levels of atherosclerotic plaque Area was significantly inhibited, but no significant change in the treatment group. At week 22, the proportion of CD4 + CD25 + FoxP3 + T cells in CD4 + T cells in peripheral blood was (4.4 ± 0.9)% in prevention group compared with that in control group [(2.6 ± 0.3)%], the difference was statistically significant (P <0.05). However, there was no significant difference in the proportion of cells treated with SEA before and after treatment (P <0.05). Conclusions SEA can reduce the level of serum total cholesterol in ApoE - / - mice. Preventing atherosclerosis by prophylactic administration of SEA can inhibit the proliferation of Treg cells induced by inflammatory cytokines in the initial stage of the disease.