论文部分内容阅读
为了探讨蓝青抗病毒合剂的体外抗菌和抗病毒作用,本研究采用连续倍比稀释法进行药物体外抑菌实验,测定几种常见细菌的最低杀菌浓度(MBC)和最低抑制浓度(MIC);采用MTT法,进行体外抗病毒实验,测定蓝青抗病毒合剂对病毒半数中毒浓度TC_(50)、半数有效浓度EC_(50)及最低有效抑菌浓度。结果表明,蓝青抗病毒合剂对实验室保存菌种金黄色葡萄球菌、甲型溶血性链球菌、大肠埃希菌的MIC分别为15.56 mg/m L、7.78 mg/m L、31.13 mg/m L;MBC分别为15.56 mg/m L、15.56 mg/m L、62.25 mg/m L。蓝青抗病毒合剂对Hela细胞的半数中毒浓度(TC_(50))为12.21 mg/m L;对MDCK细胞半数中毒浓度(TC_(50))6.25 mg/m L;对呼吸道合胞病毒的半数抑菌浓度(EC_(50))为3.74 mg/m L,在2.56 mg/m L以上的浓度时对流感病毒引起的细胞病变及血凝现象有明显抑制作用。从这里我们可以看出,蓝青抗病毒合剂具有一定的抗菌、抗病毒作用。
In order to explore the antibacterial and antiviral effects of the blue-green antiviral mixture in vitro, the antibacterial and antibacterial activities of the blue-green antiviral agent in vitro were studied in this paper. The antibacterial activity of MBC and the minimum inhibitory concentration (MIC) MTT assay was used to study the antiviral activity in vitro. The antifungal activity of Cyanobacteria was evaluated by semiquantitative analysis of TC-50, EC50 (50) and minimum inhibitory concentration (MIC). The results showed that the MICs of blue-green antiviral cocktail against laboratory-preserved Staphylococcus aureus, Streptococcus hemolyticus and Escherichia coli were 15.56 mg / m L, 7.78 mg / m L and 31.13 mg / m L and MBC were 15.56 mg / m L, 15.56 mg / m L and 62.25 mg / m L, respectively. The half concentration of TCF (TC_ (50)) was 12.21 mg / m L for Hela cells and 6.25 mg / m L for half of MDCK cells (half of the respiratory syncytial virus The bacteriostatic concentration (EC 50) was 3.74 mg / m L, and the concentration of 2.56 mg / m L significantly inhibited the cytopathic effect and blood clotting induced by influenza virus. From here we can see that blue anti-virus mixture has a certain antibacterial, anti-viral effect.