论文部分内容阅读
目的 观察知母皂苷元 (ZMS)对拟痴呆大鼠模型脑内 β 淀粉样肽沉积及胆碱能系统功能的作用。 方法 单侧基底核内联合注射 β 淀粉样肽2 5-3 5片段 (β AP2 5-3 5)和兴奋性氨基酸建立大鼠拟痴呆模型 ,然后将模型动物分为假手术组 37、模型组和ZMS组 ,采用免疫组织化学方法和图像分析法测定β AP2 5-3 5沉积斑块的面积。用避暗法及跳台法测定动物的学习记忆功能 ,用放射配基结合分析法分别测定脑胆碱乙酰转移酶 (ChAT)活性和M受体密度。结果 一次性脑内联合注射 β AP +IBO后 ,模型大鼠脑内有明显的 β AP斑块沉积 ,而模型大鼠喂服ZMS 6 0d后 ,能有效地减少β AP沉积的面积 ,同时ZMS能明显改善模型动物的学习记忆功能 ,并提高模型动物脑内ChAT活性和M受体密度。结论 ZMS可能对脑内 β AP的沉积有一定的清除作用 ,并对AD低下的胆碱能系统功能有一定的改善和治疗作用
Objective To observe the effects of timosaponin (ZMS) on brain β-amyloid peptide deposition and cholinergic system function in dementia rat models. METHODS: A rat model of dementia was established by unilateral basal nucleus injection of β-amyloid peptide 25-3 fragment (β AP2 5-3 5) and excitatory amino acids. Model animals were then divided into sham-operated group 37 and model group. In the ZMS group, the area of β AP2 5-3 5 plaques was determined by immunohistochemistry and image analysis. The animal’s learning and memory function was measured by the dark-shelter method and the step-down method. Brain choline acetyltransferase (ChAT) activity and M-receptor density were determined by radioligand binding assay. RESULTS: β-AP + IBO was injected into the brain once a time. There was a clear deposition of β AP plaques in the brains of model rats. After the model rats were fed with ZMS for 60 d, they could effectively reduce the deposition area of β AP. It can significantly improve the learning and memory function of model animals and increase the ChAT activity and M receptor density in the brain of model animals. Conclusion ZMS may have a certain clearance effect on the deposition of β-AP in the brain, and it may improve and treat the low cholinergic system function of AD.