目的比较C57BL/6小鼠肝脏、肺脏、脾脏和肠系膜淋巴结中NKT细胞的含量、亚型和功能的特点。方法分离正常C57BL/6小鼠肝脏、肺脏、脾脏和肠系膜淋巴结的淋巴细胞,利用细胞表面分子染色的方法,观察不同组织器官中CD3+NK1.1+NKT细胞及其亚型的含量;淋巴细胞经过PMA和离子霉素刺激后,应用细胞内细胞因子染色的方法,通过流式细胞仪观察NKT细胞IFN-γ、IL-4、IL-9和IL-17的产生情况。结果肝脏中NKT细胞的含量为(25.2±12)%,显著高于肺脏、脾脏和肠系膜淋巴结。肝脏、脾脏和肠系膜淋巴结中NKT细胞以CD4+细胞亚群为主,而肺脏中NKT细胞以CD4-CD8-亚群为主,同时肠系膜淋巴结的NKT细胞中存在CD4+CD8+亚群。不同组织器官中NKT细胞IFN-γ、IL-4、IL-9和IL-17产生的能力有差别。结论 C57BL/6小鼠肝脏、肺脏、脾脏和肠系膜淋巴结的NKT细胞在含量、表型和功能方面可能存在明显的差异。 Objective To compare the content, subtype and function of NKT cells in liver, lung, spleen and mesenteric lymph nodes of C57BL / 6 mice. Methods Lymphocytes in the liver, lung, spleen and mesenteric lymph nodes of normal C57BL / 6 mice were isolated and the contents of CD3 + NK1.1 + NKT cells and their subtypes in different tissues and organs were observed by cell surface molecular staining. Lymphocytes After stimulation with PMA and ionomycin, the production of IFN-γ, IL-4, IL-9 and IL-17 in NKT cells was observed by flow cytometry after intracellular cytokine staining. Results The content of NKT cells in liver was (25.2 ± 12)%, which was significantly higher than that in lung, spleen and mesenteric lymph nodes. NKT cells in the liver, spleen and mesenteric lymph nodes were predominantly CD4 + cell subsets, while NKT cells in the lungs were predominantly CD4 + CD8 + subsets and CD4 + CD8 + subsets in NKT cells of the mesenteric lymph nodes. There is a difference in the ability of NKT cells to produce IFN-γ, IL-4, IL-9 and IL-17 in different tissues and organs. Conclusion NKT cells in liver, lung, spleen and mesenteric lymph nodes of C57BL / 6 mice may be significantly different in content, phenotype and function.