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为提高检测土拉弗氏菌的特异性和敏感性，建立了土拉菌ＰＣＲ及核酸杂交检测方法。运用平板计数、多聚酶链反应对土拉菌气溶胶稳定性进行了比较，结果表明ＰＣＲ具有较高灵敏度，并且在采样后３小时ＰＣＲ就可以得出定性结果，而平板计数则需要３～７天。采用ＰＣＲ法合成了土拉菌３７６－ｂｐ探针，分别对细菌菌液、５６８－ｂｐＰＣＲ产物和气溶胶样品进行杂交，结果表明菌悬液直接杂交可检出１０５ＣＦＵ左右的细菌，检测ＰＣＲ产物可达４０ｐｇ。ＰＣＲ和Ｓｏｕｔｈｅｒｎ印迹相结合有利于细菌的分离鉴定
In order to improve the specificity and sensitivity of detection of Tulalura, PCR and nucleic acid hybridization detection methods were established. The plate count and polymerase chain reaction were used to compare the stability of T. aeruginosa. The results showed that PCR had higher sensitivity, and the qualitative results could be obtained by PCR three hours after sampling, while the plate counting required three to seven days . The 376-bp probe of Tulara was synthesized by PCR, and the bacterial and 568-bp PCR products were respectively hybridized with the aerosol sample. The results showed that the bacterial suspension could detect about 105CFU of bacteria and detect the PCR products 40pg. The combination of PCR and Southern blot favors the isolation and identification of bacteria