Wake-promoting effects of vagus nerve stimulation after traumatic brain injury:upregulation of orexi

来源 :中国神经再生研究(英文版) | 被引量 : 0次 | 上传用户:allsky_
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
Orexins, produced in the lateral hypothalamus, are important neuropeptides that participate in the sleep/wake cycle, and their expres-sion coincides with the projection area of the vagus nerve in the brain. Vagus nerve stimulation has been shown to decrease the amounts of daytime sleep and rapid eye movement in epilepsy patients with traumatic brain injury. In the present study, we investigated whether vagus nerve stimulation promotes wakefulness and affects orexin expression. A rat model of traumatic brain injury was established using the free fall drop method. In the stimulated group, rats with traumatic brain injury received vagus nerve stimulation (frequency, 30 Hz;current, 1.0 mA; pulse width, 0.5 ms; total stimulation time, 15 minutes). In the antagonist group, rats with traumatic brain injury were intracerebroventricularly injected with the orexin receptor type 1 (OX1R) antagonist SB334867 and received vagus nerve stimulation. Changes in consciousness were observed after stimulation in each group. Enzyme-linked immunosorbent assay, west blot assay and immunohistochemistry were used to assess the levels of orexin-A and OX1R expression in the prefrontal cortex. In the stimulated group, consciousness was substantially improved, orexin-A protein expression gradually increased within 24 hours after injury and OX1R expres-sion reached a peak at 12 hours, compared with rats subjected to traumatic brain injury only. In the antagonist group, the wake-promoting effect of vagus nerve stimulation was diminished, and orexin-A and OX1R expression were decreased, compared with that of the stim-ulated group. Taken together, our findings suggest that vagus nerve stimulation promotes the recovery of consciousness in comatose rats after traumatic brain injury. The upregulation of orexin-A and OX1R expression in the prefrontal cortex might be involved in the wake-promoting effects of vagus nerve stimulation.
其他文献
Multiple sclerosis (MS) is a chronic inflammatory and neurodegenerative disorder that is thought to be mediated by autoreactive T lymphocytes that find their wa
期刊
Traumatic brain injury (TBI) is a mechanical injury to brain tissue that leads to an impairment of function and a broad spectrum of symptoms and disabilities; o
期刊
期刊
The basal ganglia (BG) assemble a series of deep gray matter structures forming recurrent loops that include the cortex and thalamus, and that participate in th
期刊
【摘要】目的对门诊患者进行及时随访是降低患者再入院率的关键策略。在出院后 30 d 内再入院患者中,有一半未在出院后进行随访。对于病情多变且复杂的患者,应通过指导确定出
期刊
目的从东方田鼠血清中分离纯化与其天然抗日本血吸虫感染特性相关的蛋白质.方法以分子筛色谱及高效液相色谱技术对东方田鼠血清蛋白质进行分离和纯化,以体外日本血吸虫童虫杀
目的 检测前列腺癌PC3细胞中泛素连接酶NEDD4-1 (neural precursor cell expressed developmentally downregulated 4-1,NEDD4-1)对酪氨酸蛋白激酶A(tyrosine kinase A,TrkA)
目的了解鼠类自然感染汉坦病毒(HV)时间动态变化. 方法选取北京昌平南口镇某部驻地,从2002年8月至2004年5月采用夹夜法捕获鼠形动物,针对汉坦病毒 M基因部分片段设计SEOV和HT
Ischemic brain injury triggers neuronal cell death by apoptosis via caspase activation and by necroptosis through activation of the receptor-interacting protein
Heat-sensitive suspended moxibustion has a neuroprotective effect against focal cerebral ischemia/reperfusion injury, but the underly-ing mechanisms remain uncl