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胎肝组织中存在低分子肿瘤抑制物。胎肝细胞悬液或裂解液用甲醇－丙酮提取后保留有大部分抑瘤活性。体外液体培养条件下以其对人急性粒系白血病细胞系（ＨＬ－６０）的抑制作用为指标，跟踪活性成分的分离过程。提取物经反相Ｃ１８中压液相色谱、ＳｅｐｈａｄｅｘＬＨ－２０凝胶过滤色谱及正相高压液相色谱分离得到两种活性物质，经核磁共振和高分辨率质谱仪鉴定为７－酮基胆固醇（７－ｋｅｔｏｃｈｏｌｅｓｔｅｒｏｌ，７－ＫＣ）和７β－羟基胆固醇（７β－ｈｙｄｒｏｘｙｃｈｏｌｅｓｔｅｒｏｌ，７β－ＨＣ）。７－ＫＣ和７β－ＨＣ对ＨＬ－６０细胞增殖较对正常人骨髓粒－巨细胞集落形成单位有更强的抑制作用
There are low molecular tumor suppressors in fetal liver tissue. Fetal liver cell suspension or lysate retained most of the tumor-inhibitory activity after being extracted with methanol-acetone. The inhibitory effect of HL-60 on human acute myelocytic leukemia cell lines (HL-60) was monitored under in vitro liquid culture conditions to follow the separation of the active ingredients. The extracts were separated by reverse phase C18 medium pressure liquid chromatography, Sephadex LH-20 gel filtration chromatography and normal phase high pressure liquid chromatography to obtain two kinds of active substances, which were identified as 7-ketocholesterol by nuclear magnetic resonance and high resolution mass spectrometer ( 7-ketocholesterol, 7-KC) and 7β-hydroxycholesterol (7β-HC). 7-KC and 7β-HC inhibit HL-60 cell proliferation more strongly than normal human bone marrow-macrophage colony-forming units