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目的探讨急性白血病（ＡＬ）多药耐药基因（ｍｄｒ１）及多药耐药相关蛋白基因（ＭＲＰ）的表达与预后的关系。方法采用逆转录聚合酶链反应（ＲＴＰＣＲ）的方法检测５５例ＡＬ患者的ｍｄｒ１及ＭＲＰ的表达。结果发现复发急变组的ｍｄｒ１及ＭＲＰ基因表达水平（０．７３５±０．２４９，１．１５７±０．４４７）较初治组（０．４０８±０．１８６，０．４６５±０．２５３）明显升高（Ｐ＜０．０１）。而且ｍｄｒ１及ＭＲＰ同时高表达者的完全缓解（ＣＲ）率明显低于ｍｄｒ１及ＭＲＰ同时低表达者（Ｐ＜０．０１）。结论ｍｄｒ１和ＭＲＰ同时高表达是判断ＡＬ患者耐药复发及不良预后的重要因素。
Objective To investigate the relationship between the expression of multidrug resistance gene (mdr1) and multidrug resistance-associated protein gene (MRP) in acute leukemia (AL) and its prognosis. Methods The expression of mdr1 and MRP in 55 patients with AL were detected by reverse transcriptase polymerase chain reaction (RT-PCR). The results showed that the expression levels of mdr1 and MRP genes in the recurrent blast crisis group (0.735±0.249,1.157±0.447) were higher than those in the newly treated group (0.408±0.186, 0.465±0.253). Significantly increased (P <0.01). And the complete remission (CR) rate of mdr1 and MRP were significantly lower than those of mdr1 and MRP (P<0.01). Conclusion The high expression of mdr1 and MRP at the same time is an important factor to determine the relapse and poor prognosis of AL patients.