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[目的]建立一种高效液相色谱分析方法,同时测定马铃薯及其土壤中的霜脲氰和嘧菌酯。[方法]样品用乙腈提取,CARB-NH2固相萃取柱净化后,以甲醇-水(体积比60:40)为流动相,流速为0.8 mL/min,使用BDS-C18色谱柱和紫外检测器,在254nm检测波长下,对霜脲氰和嘧菌酯进行分离检测。[结果]霜脲氰和嘧菌酯在0.05~2.0mg/L范围内线性良好,相关系数分别为0.999 8和0.999 9。在0.05~1.0 mg/kg添加水平下,霜脲氰的平均回收率为84.7%~94.2%,变异系数为1.0%~9.2%;嘧菌酯的平均回收率为94.0%~100.5%,变异系数为0.9%~6.9%。方法的最低检测质量浓度为0.125 mg/L。[结论]该方法简单、快捷、准确度和精密度高,适用于马铃薯及其土壤中霜脲氰和嘧菌酯的检测,也为霜脲氰和嘧菌酯在食品以及其他农产品中的监测提供参考。
[Objective] The research aimed to establish a HPLC method for simultaneous determination of cymoxanil and azoxystrobin in potatoes and their soils. [Method] The sample was extracted with acetonitrile and purified by CARB-NH2 solid phase extraction column. The mobile phase consisted of methanol and water (60:40 by volume) with a flow rate of 0.8 mL / min. The sample was separated on a BDS-C18 column and UV detector , At 254nm detection wavelength, the cymoconazole and azoxystrobin separation and detection. [Result] Cymoconazole and azoxystrobin had a good linearity in the range of 0.05-2.0 mg / L with correlation coefficients of 0.999 8 and 0.999 9, respectively. The average recoveries of azoxystrobin ranged from 84.7% to 94.2% and the coefficients of variation ranged from 1.0% to 9.2% at the level of 0.05-1.0 mg / kg. The average recoveries of azoxystrobin ranged from 94.0% to 100.5% 0.9% ~ 6.9%. The minimum detectable concentration of the method was 0.125 mg / L. [Conclusion] The method was simple, rapid, accurate and precise and suitable for the determination of cymoxanil and azoxystrobin in potato and its soil as well as the monitoring of cymoxanil and azoxystrobin in food and other agricultural products for reference.