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[目的]探讨细丝蛋白A(filamin A,FLNA)在前列腺癌中的表达及意义。[方法]采用免疫组织化学方法、Western blot检测68例前列腺癌组织及距其癌组织边缘2cm以上的镜下未见癌浸润的正常组织中FLNA蛋白的表达情况。[结果]免疫组织化学结果表明,FLNA蛋白在前列腺癌组织的表达阳性率为38.2%,较正常前列腺组织(75.7%)明显降低(P<0.05)。Western blot结果表明,FLNA的蛋白在前列腺癌组织的相对表达量较正常前列腺组织明显降低,两者比较差异具有统计学意义(P<0.05)。FLNA蛋白表达与前列腺患者年龄、PSA水平无关(P>0.05);而与淋巴结转移、临床分期以及Gleason评分有关(P<0.05)。[结论]前列腺癌组织中FLNA蛋白表达明显减低,可能在前列腺癌发生的负反馈调节中起重要作用。
[Objective] To investigate the expression and significance of filamin A (FLNA) in prostate cancer. [Method] Immunohistochemistry and Western blot were used to detect the expression of FLNA protein in 68 cases of prostate cancer tissues and normal tissues without carcinoma infiltrating more than 2 cm from the edge of their cancer tissues. [Results] Immunohistochemistry results showed that the positive rate of FLNA protein expression in prostate cancer was 38.2%, which was significantly lower than that in normal prostate tissue (75.7%) (P <0.05). Western blot results showed that the relative expression level of FLNA protein in prostate cancer tissues was significantly lower than that in normal prostate tissues (P <0.05). The expression of FLNA was not related to the age and PSA level of patients with prostate cancer (P> 0.05), but correlated with lymph node metastasis, clinical stage and Gleason score (P <0.05). [Conclusion] The expression of FLNA protein in prostate cancer is significantly reduced, which may play an important role in the negative feedback regulation of prostate cancer.