论文部分内容阅读
目的:探讨细胞色素P450 19基因多态性与新疆维吾尔族女性乳腺癌相关关系。方法:应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测112例维吾尔族女性乳腺癌患者(病例组)和139例维吾尔族正常女性(对照组)细胞色素P450 19基因rs10046位点多态性,分析该位点多态性与新疆维吾尔族女性乳腺癌易感性的关系。结果:细胞色素P450(CYP)19基因rs10046位点存在C-T的替换。等位基因C、T在病例组分布频率为48.2%、51.8%,对照组频率为47.5%、52.5%,两组等位基因分布无统计学差异(P>0.05)。其中CC、TC、TT基因型在病例组分布频率分别为26.8%、42.9%、30.4%,在对照组中分布频率分别为18.0%、59.0%、23.0%,两组间分布差异有统计学差异(P<0.05)。BMI≥25、年龄≥50岁、活产次数≥2次为维吾尔族乳腺癌发病的危险因素,流产、细胞色素P450 19 rs10046位点TC基因型为维吾尔族乳腺癌发病的保护因素。校正年龄、BMI、月经初潮年龄、怀孕、哺乳乳腺癌发病危险因素后,绝经为维吾尔族乳腺癌发病的独立危险因素。结论:新疆维吾尔族女性乳腺癌的发病与年龄、BMI、活产次数、流产及细胞色素P450 19rs10046位点多态性密切相关,其中携带TC基因型和流产可降低维吾尔族女性乳腺癌的发病风险。
Objective: To investigate the relationship between cytochrome P450 19 gene polymorphism and breast cancer in Xinjiang Uygur women. METHODS: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to detect cytochrome P450 19 gene in 112 Uygur women with breast cancer (case group) and 139 Uygur normal women (control group). Polymorphism of rs10046 locus was used to analyze the relationship between polymorphism of this locus and breast cancer susceptibility in Xinjiang Uygur women. RESULTS: There was a C-T substitution at the rs10046 locus of the cytochrome P450 (CYP) 19 gene. The frequency of alleles C and T in the case group was 48.2% and 51.8%, and that of the control group was 47.5% and 52.5%. There was no significant difference in allele distribution between the two groups (P>0.05). The frequencies of CC, TC, and TT genotypes in the case group were 26.8%, 42.9%, and 30.4%, respectively, and those in the control group were 18.0%, 59.0%, and 23.0%, respectively. There was a statistically significant difference between the two groups. (P<0.05). BMI ≥ 25, age ≥ 50 years, and live birth frequency ≥ 2 times were risk factors for the onset of Uighur breast cancer. Abortion, cytochrome P450 19 rs10046 loci TC genotypes were protective factors for the onset of Uighur breast cancer. After adjusting for age, BMI, age at menarche, risk factors for pregnancy, and breast cancer, menopause was an independent risk factor for the onset of Uighur breast cancer. Conclusion: The incidence of breast cancer in Uygur females in Xinjiang is closely related to age, BMI, number of live births, abortion and polymorphism of cytochrome P450 19rs10046 loci. Among them, TC genotype and abortion can reduce the risk of breast cancer in Uygur females. .