论文部分内容阅读
目的探讨子宫内膜癌中同源盒基因A10(HOXA10)及p21基因表达的相关性。方法提取子宫内膜癌及正常子宫内膜的mRNA及总蛋白,使用RT-PCR检测HOXA10及p21基因表达,Western blot检测HOXA10蛋白表达,分析HOXA10及p21基因表达相关性。结果HOXA10及p21基因在子宫内膜癌中表达下调,两者之间表达具有正相关。HOXA10蛋白随着肿瘤等级上升表达呈下降趋势。结论子宫内膜癌中HOXA10、p21基因的低表达可能参与子宫内膜癌的进展。
Objective To investigate the correlation between HOXA10 gene and p21 gene expression in endometrial carcinoma. Methods The mRNA and total protein of endometrial carcinoma and normal endometrium were extracted. The expressions of HOXA10 and p21 were detected by RT-PCR. The expression of HOXA10 protein was detected by Western blot. The correlation between HOXA10 and p21 gene expression was analyzed. Results HOXA10 and p21 genes were down-regulated in endometrial carcinoma with a positive correlation between them. HOXA10 protein showed a decreasing trend with the increase of tumor grade. Conclusion The low expression of HOXA10 and p21 in endometrial carcinoma may be involved in the progression of endometrial carcinoma.