整体染色:(1)在10毫升福末林(普通的);45毫升95％乙醇;2毫升冰醋酸組成的混合剂內固定24—48小时。(2)不用洗滌,轉入70％乙醇內3—10天。(3)在蒸餾水內洗滌一小时。(4)在50℃下,在10％硫酸銅(CuSO_4·5H_2O)內浸3小时。(5)不經洗滌,在50℃下,轉入1％硝酸銀內1.0—1.5小时。(6)在蒸餾水內洗3分钟,連續三次,每次一分钟。(7)在40—45℃时水浴內,在下述显影剂內还原,显影剂配法:1％連苯三酚10毫升;56％乙醇(一般为60％) Overall Dyeing: (1) Fix for 24 - 48 hours in 10 ml of Forosin (normal); 45 ml of 95% ethanol; 2 ml of glacial acetic acid. (2) without washing, transferred to 70% ethanol 3-10 days. (3) Wash in distilled water for one hour. (4) Dip in 10% copper sulfate (CuSO 4 · 5H 2 O) for 3 hours at 50 ° C. (5) without washing, at 50 ℃, into 1% silver nitrate 1.0-1.5 hours. (6) Wash in distilled water for 3 minutes, three times in a row, one minute at a time. (7) at 40-45 ° C in a water bath, reducing agents within the following developer: 1% pyrogallol 10 ml; 56% ethanol (typically 60%)