植物生长物质对不同烘烤工艺下烟叶多酚含量及其酶活性的影响

来源 :河南农业科学 | 被引量 : 0次 | 上传用户:enlic
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为了筛选促进烟叶生长的植物生长物质,研究了烤烟喷施植物生长物质(主要成分为赤霉素)对烘烤过程(正常和高温高湿条件下)中烟叶含水量、多酚氧化酶活性、多酚含量等指标的影响。结果表明,正常和非正常(高温高湿)2种烘烤条件下,植物生长物质处理烟叶的含水量差异较大,正常烘烤条件下,烘烤0~36h烟叶含水量下降速度缓慢,而在后期下降速度较快;而非正常烘烤条件下,烘烤0~60h烟叶含水量下降均较慢,至烘烤60h,仍在鲜烟叶的50%左右。在正常烘烤条件下,多酚氧化酶活性随烘烤时间的延长持续下降,到烘烤60h时,活性达到最低;而在非正常烘烤条件下,多酚氧化酶活性呈先降低后升高再降低趋势,但在烘烤最后酶活性仍明显高于正常烘烤条件下的酶活性。2种烘烤条件下,各处理多酚含量总体上均有升高趋势,且在烘烤前期,除少数处理外,喷施植物生长物质处理能提高烟叶多酚含量,但到烘烤后期大部分处理间差异不明显。2种烘烤条件下,喷施植物生长物质各处理上等烟比例均显著高于CK,且正常烘烤条件下的上等烟比例均高于非正常烘烤条件,可见,施用植物生长物质均提高了上等烟比例,以植物生长物质4号和7号的配方效果较好。 In order to screen the plant growth substances that promote the growth of tobacco leaves, the effect of spraying plant growth substance (mainly GA3) on the leaf water content, polyphenol oxidase activity, Polyphenol content and other indicators. The results showed that under normal and abnormal conditions (high temperature and high humidity), the moisture content of tobacco leaves treated by plant growth substances varied greatly. Under normal conditions, the moisture content of tobacco leaves decreased slowly from 0 to 36 hours The rate of decline in the latter part of the faster; but not normal baking conditions, 0 ~ 60h baking leaf water content decreased slowly, to 60h baking, fresh leaves are still about 50%. Under normal baking conditions, the activity of polyphenol oxidase decreased with the prolongation of baking time, and reached the lowest level at 60h after baking. However, the polyphenol oxidase activity decreased first and then increased under the abnormal baking conditions High and then reduce the trend, but at the end of the baking enzyme activity was significantly higher than normal baking conditions enzyme activity. Under the two kinds of roasting conditions, the content of polyphenols in all treatments tended to increase. In the early stage of roasting, except for a few treatments, the spraying of plant growth substances could increase the content of polyphenols in tobacco leaves, Some differences between the treatment is not obvious. Under the two kinds of roasting conditions, the proportion of top-quality tobacco plants sprayed with plant growth substances was significantly higher than that of CK, and the proportion of superior tobacco under normal roasting conditions was higher than that of non-normal roasting conditions. It can be seen that the application of plant growth substances The proportion of top-grade tobacco was increased, and the plant growth substances No. 4 and No. 7 had better formulation effects.
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