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目的:建立HPLC法同时测定心宁片中芦丁、丹酚酸B、人参皂苷Rb1含量的方法。方法:采用CAPCELL PAK MG(250 mm×4.6 mm,5μm)色谱柱,流动相为乙腈(A)-0.1%磷酸溶液(B),梯度洗脱,流速1.0 ml·min~(-1),柱温35℃,检测波长为354 nm、286 nm和203 nm(波长切换方式)。结果:芦丁浓度在0.027 3~1.360 0 mg·ml~(-1)范围内(r=1.000 0),丹酚酸B浓度在0.024 4~1.220 0 mg·ml~(-1)范围内(r=1.000 0),人参皂苷Rb_1浓度在0.018 6~0.931 0 mg·ml~(-1)范围内(r=0.999 9),与峰面积均呈良好的线性关系;平均回收率分别为102.3%,98.7%,101.7%,RSD分别为1.1.%,0.8%,1.8%(n=6)。结论:本文建立HPLC法可实现同时测定芦丁、丹酚酸B、人参皂苷Rb13种有效成分,方法快速、简便、结果准确,可为心宁片提供更全面、可靠的质量控制方法。
Objective: To establish a HPLC method for simultaneous determination of rutin, salvianolic acid B and ginsenoside Rb1 in Xinning Tablet. Methods: A CAPCELL PAK MG (250 mm × 4.6 mm, 5 μm) column was used. The mobile phase consisted of acetonitrile - 0.1% phosphoric acid solution (B) with gradient elution at a flow rate of 1.0 ml · min ~ Temperature 35 ℃, detection wavelength of 354 nm, 286 nm and 203 nm (wavelength switching mode). Results: The concentration of rutin in the range of 0.027 3 ~ 1.360 0 mg · ml ~ (-1) (r = 1.000 0) and the concentration of salvianolic acid B in the range of 0.024 4 ~ 1.220 0 mg · ml ~ (-1) r = 1.000 0). The concentration of ginsenoside Rb_1 in the range of 0.018 6-0.93 0 mg · ml -1 (r = 0.999 9) showed a good linear relationship with the peak area. The average recoveries were 102.3% , 98.7% and 101.7%, respectively. The RSDs were 1.1%, 0.8% and 1.8% respectively (n = 6). Conclusion: The HPLC method can be used to determine the active components of rutin, salvianolic acid B and ginsenoside Rb simultaneously. The method is rapid, simple and accurate. It can provide a more comprehensive and reliable quality control method for Xinning Tablet.