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目的:观察桑精胶囊对2型糖尿病大鼠脂肪和骨骼肌组织胰岛素受体底物1(insulin receptor substrat1,IRS-1)蛋白表达及其丝氨酸磷酸化水平的影响,探讨其治疗2型糖尿病的分子机制。方法:采用尾静脉注射小剂量链脲佐菌素(strepto-zotocin,STZ)加高脂高热卡饲料喂养的方法建立2型糖尿病(type2diabetes mellitus,T2DM)大鼠模型,将造模动物随机分为模型组、二甲双胍组和桑精胶囊组,另设正常对照组。药物干预8周后,用免疫沉淀和Western印迹方法检测脂肪和骨骼肌组织胰岛素刺激前和胰岛素刺激后IRS-1表达水平和丝氨酸磷酸化水平。结果:与模型组比较,桑精胶囊组胰岛素刺激前和胰岛素刺激后脂肪和骨骼肌组织中IRS-1蛋白表达均增高,IRS-1丝氨酸磷酸化水平均显著下降(P<0.05或P<0.01)。结论:桑精胶囊对2型糖尿病的治疗效应可能与提高脂肪和骨骼肌组织中IRS-1蛋白表达并抑制其丝氨酸磷酸化水平有关。
Objective: To observe the effect of Sangjing Capsule on the protein expression of insulin receptor substrat1 (IRS-1) and its serine phosphorylation in type 2 diabetic rats and to explore its effect on type 2 diabetes mellitus Molecular mechanism. Methods: A rat model of type 2 diabetes mellitus (T2DM) was established by injecting a small dose of streptozotocin (STZ) into the tail vein and feeding with high fat and high calorie diet. The model animals were randomly divided into Model group, metformin group and Sang Jing Capsule group, another set of normal control group. After 8 weeks of drug intervention, the expression of IRS-1 and serine phosphorylation were measured before and after insulin stimulation of fat and skeletal muscle by immunoprecipitation and Western blotting. Results: Compared with the model group, the IRS-1 protein expression in IRS-1 and IRS-1 serine phosphorylation of Sangjing capsule group were significantly decreased (P <0.05 or P <0.01) ). Conclusion: The effect of Sangjing capsule on type 2 diabetes mellitus may be related to the increase of IRS-1 protein expression and the inhibition of its serine phosphorylation in fat and skeletal muscle.