根据杂交种带型双亲互补的原则,从30对SSR引物中筛选出适合北京市主栽西瓜品种的纯度鉴定引物,其中京欣1号和暑宝可鉴定的引物最多,达到11对,京秀可鉴定引物最少,只有2对。结合种仁DNA快速提取方法,利用筛选的引物对已知纯度混杂样品进行鉴定,结果显示SSR分子检测方法可以准确鉴定掺杂的亲本,可用于西瓜杂交品种纯度准确、经济、快速鉴定。 According to the principle of reciprocal complementarity of hybrids, we selected 30 pairs of SSR primers to identify the purity primers suitable for the cultivated watermelon varieties in Beijing. Among them, Jingxin No.1 and Shucuo identified the most of the primers, reaching 11 pairs, Jingxiu Primer can be identified at least, only two pairs. The results showed that the SSR molecular detection method can accurately identify the doping parents and can be used for the accurate, economical and rapid identification of the hybrids of watermelon.