论文部分内容阅读
目的观察了两种模拟飞行条件（１５００ ｍ 轻度低压缺氧和５５００ ｍ 中度低压吸氧重复暴露）下小鼠多脏器自由基代谢变化的特点。方法昆明种小鼠６０ 只，随机分为６ 组（ｎ＝１０）进行缺氧与吸氧实验，实验完毕后，取小鼠尾血做血常规检查。次日，将小鼠断头处死，取脑、心、肺、肝、肾制备匀浆，测定丙二醛（ＭＤＡ） 含量和超氧化物歧化酶（ＳＯＤ）活性。结果１５００ ｍ 轻度低压缺氧重复暴露８ ｗｋ 使肺ＭＤＡ含量显著增高，提示引起小鼠肺的自由基损伤；５５００ ｍ 中度低压吸氧重复暴露８ ｗｋ 后，心肌ＭＤＡ含量、ＳＯＤ活性均明显增高，提示引起小鼠心肌的自由基损伤。结论模拟飞行缺氧与吸氧可以导致小鼠肺及心肌的自由基损伤。
Objective To observe the changes of free radicals metabolism in multiple organs of mice under two simulated flight conditions (mild hypobaric hypoxia at 1500 m and repeated hypobaric exposure at 5500 m). Methods Sixty Kunming mice were randomly divided into six groups (n = 10) for hypoxia and oxygen inhalation experiments. After the experiment was completed, the tail blood of mice was used for blood routine examination. The next day, the mice were decapitated and the brain, heart, lung, liver and kidney were homogenized to determine the content of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD). Results Repeated exposure to light hypobaric hypoxia at 1500 m for 8 wk resulted in a significant increase of MDA content in the lung, which suggested that free radical damage was induced in the lung of mice. Repeated exposure to 5500 m oxygen for 8 weeks significantly increased MDA content and SOD activity Increased, suggesting that caused by the mouse heart muscle free radical damage. Conclusion Simulated flight hypoxia and oxygen inhalation can lead to free radical damage in lung and myocardium of mice.