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目的通过基于气相色谱-质谱联用(GC-MS)的代谢组学技术检测小鼠RSV肺炎对肝脏的损害,阐明黄芩素保护肝损害的作用机制。方法 RSV滴鼻感染BALB/c小鼠建立RSV肺炎模型,予黄芩素混悬液灌胃干预,并于滴鼻7d后处死各组小鼠,取肝组织,运用GC-MS检测肝组织内源性代谢物及其相对含量的变化,分析其可能参与的代谢通路。结果 RSV肺炎小鼠的肝脏组织中共测得31种内源性代谢产物,其中天冬氨酸、甘氨酸、延胡索酸、苹果酸、肌醇、棕榈酸、肌苷、硬脂酸、胆固醇、松醇、麦芽糖等为差异代谢产物(P<0.05),主要与天冬氨酸、甘氨酸、磷酸肌醇代谢通路有关。结论运用基于GC-MS的代谢组学技术能从微观角度检测RSV肺炎对肝脏的损害,黄芩素可以改善肝脏代谢功能,从而发挥保护作用。
OBJECTIVE: To detect the damage of liver caused by RSV pneumonia in mice based on gas chromatography-mass spectrometry (GC-MS) and to elucidate the mechanism of protection of baicalein on liver damage. Methods BALB / c mice were intranasally infected with RSV to establish a model of RSV pneumonia. The mice were intragastrically administrated with baicalein and intraperitoneally. The mice were sacrificed 7 days after the intranasal nasal instillation, and the liver tissues were harvested. The liver tissues were harvested by GC-MS Sex metabolites and their relative content changes, analysis of their possible metabolic pathways. Results A total of 31 endogenous metabolites were detected in liver tissues of mice with RSV pneumonia, including aspartic acid, glycine, fumaric acid, malic acid, inositol, palmitic acid, inosine, stearic acid, cholesterol, Maltose and other metabolites as the difference (P <0.05), mainly with aspartate, glycine, phosphoinositide metabolic pathway. Conclusion GC-MS-based metabonomics can detect liver damage caused by RSV pneumonia from a microscopic point of view. Baicalein can improve hepatic metabolism and thus play a protective role.