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目的评价龈下菌斑微生物表面抗原对人牙龈上皮细胞(HGECs)白细胞介素-1β(IL-1β)及基质金属蛋白酶-8(MMP-8)表达的影响。方法收集慢性牙周炎患者的龈下菌斑,经γ射线灭活制备表面抗原,按不同浓度稀释分为B、C、D、E、F组,A组为空白对照组。刺激人牙龈上皮细胞24 h后,采用ELISA检测细胞培养液中IL-1β和MMP-8含量,统计分析各组细胞因子的变化。结果龈下菌斑表面抗原刺激HGECs 24 h后,两种细胞因子表达的平均水平明显高于空白对照组(P<0.05)。IL-1β和MMP-8随抗原浓度的增加而升高,但达到峰值后下降。结论γ射线灭活可有效保留龈下菌斑微生物表面抗原;在细菌尚未侵入牙龈上皮细胞时,龈下菌斑表面抗原已促使细胞表达IL-1β和MMP-8,诱导牙周组织的炎症和免疫反应。
Objective To evaluate the effects of subgingival plaque microbe surface antigen on the expression of interleukin-1β (IL-1β) and matrix metalloproteinase-8 (MMP-8) in human gingival epithelial cells (HGECs). Methods The subgingival plaque in patients with chronic periodontitis was collected, and the surface antigens were prepared by γ-ray inactivation. The antigens were divided into groups B, C, D, E and F according to different concentrations. A group was blank control group. After stimulating human gingival epithelial cells for 24 h, the contents of IL-1β and MMP-8 in the cell culture medium were detected by ELISA, and the changes of cytokines in each group were statistically analyzed. Results The average level of expression of two cytokines in HGECs stimulated by subgingival plaque surface antigen for 24 h was significantly higher than that of the blank control group (P <0.05). IL-1βand MMP-8 increased with the increase of antigen concentration, but decreased after reaching the peak. Conclusions γ-ray inactivation can effectively preserve the subgingival plaque microbe surface antigen. When the bacteria have not invaded into the gingival epithelial cells, the subgingival plaque surface antigens have induced the expression of IL-1β and MMP-8 in the cells and induced the inflammation of the periodontal tissues and immune response.