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目的优选通络调脂胶囊的水提工艺。方法采用正交试验设计,用高效液相色谱法(HPLC)同时测定黄芩苷、芍药苷、葛根素3种指标成分的含量。色谱柱:Agilent ZORBAX Eclispse SB-C18柱(4.6 mm×250 mm,5μm);色谱条件:流动相乙腈-0.1%磷酸水梯度洗脱,流速1.0 m L·min-1,检测波长为280 nm(黄芩苷)、230nm(芍药苷)、250 nm(葛根素)。结果根据回归方程黄芩苷、芍药苷和葛根素分别在0.3336~2.0016μg·m L-1、0.1322~0.7932μg·m L-1、0.0938~0.5628μg·m L-1范围内进样量与峰面积之间线性关系均良好;3个成分的平均回收率分别为100.9%、103.0%和98.3%。最佳提取工艺为加6倍量水,提取3次,每次1 h;工艺验证黄芩苷、芍药苷、葛根素转移率分别为92.83%、87.98%、86.83%,浸膏得率为33.80%。结论采用多指标综合评分法优选通络调脂胶囊的提取工艺,方法简便,准确可靠。
Objective To optimize Tongluozhuan capsule water extraction process. Methods Orthogonal design was used to determine the content of baicalin, paeoniflorin and puerarin simultaneously by high performance liquid chromatography (HPLC). Column: Agilent ZORBAX Eclispse SB-C18 column (4.6 mm × 250 mm, 5 μm). The chromatographic conditions were as follows: gradient elution with mobile phase of acetonitrile-0.1% phosphoric acid and the flow rate of 1.0 m L · min- Baicalin), 230 nm (paeoniflorin), 250 nm (puerarin). Results According to regression equation baicalin, paeoniflorin and puerarin in the range of 0.3336 ~ 2.0016μg · m L-1, 0.1322 ~ 0.7932μg · m L-1, 1.0938 ~ 0.5628μg · m L-1, respectively, The linear relationship between the three areas was good. The average recoveries of three components were 100.9%, 103.0% and 98.3% respectively. The optimum extraction process was adding 6 times amount of water and extracting 3 times for 1 h each time. The conversion rates of baicalin, paeoniflorin and puerarin were 92.83%, 87.98% and 86.83% respectively, the extraction yield was 33.80% . Conclusion The multi-index comprehensive score method is adopted to optimize the extraction process of Tongluo Tiaozhi Capsule. The method is simple, accurate and reliable.