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为了探明不同变应原与气道粘附分子之间的关系,本研究采用屋尘螨(house dust mite,HDM)和卵蛋白(ovalbumin,OVA)分别致敏BABL/c小鼠,采用Buxco小动物肺功能分析系统观察气道的反应性,计数外周血白细胞数目,肺部HE染色观察气道的病理改变,利用免疫组织化学技术检测气道上皮粘附分子整合素β4(integrinβ4,ITGB4)和细胞间粘附分子-1(intercellular adhesion molecule-1,ICAM-1)的表达。结果显示,与对照组比较,HDM组和OVA组小鼠外周血白细胞数目明显增加,肺部炎症细胞浸润,气道上皮ITGB4表达下调,而ICAM-1表达上调。然而,OVA组小鼠气道上皮ICAM-1的表达上调较HDM组更为明显,炎症细胞的浸润也更严重。采用si RNA沉默16HBE人支气管上皮细胞株ITGB4的表达后,ICAM-1的表达上调。以上结果均提示,变应原能够通过改变气道粘附分子谱来影响肺内炎症反应。
In order to investigate the relationship between different allergens and airway adhesion molecules, BABL / c mice were sensitized with house dust mite (HDM) and ovalbumin (OVA) The lung function analysis system of small animals was used to observe the airway reactivity. The number of peripheral white blood cells was counted. The pathological changes of the airways were observed by HE staining. The expressions of integrin β4 (ITGB4), airway epithelial adhesion molecules And intercellular adhesion molecule-1 (ICAM-1). The results showed that compared with the control group, the number of leukocytes in peripheral blood of HDM group and OVA group was significantly increased. The infiltration of inflammatory cells in the lung and the expression of ITGB4 in airway epithelium were down-regulated while the expression of ICAM-1 was up-regulated. However, up-regulation of ICAM-1 expression in airway epithelium of OVA group was more obvious than that of HDM group, and infiltration of inflammatory cells was more serious. The expression of ICAM-1 was upregulated by si RNA silencing of ITGB4 in 16HBE human bronchial epithelial cell line. The above results suggest that allergen can change the airway adhesion molecule spectrum to affect the inflammatory reaction in the lung.