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目的:建立细辛饮片中马兜铃酸A的痕量测定方法。方法:采用UPLC-UV法,色谱柱为Acquity UPLCTM HSS T3色谱柱(2.1mm×50mm,1.8μm);流动相为乙腈(A)-0.1%甲酸(B),梯度洗脱(0min,30%A;5min,60%A;7min,100%A),流速0.5mL.min-1;检测波长251nm;柱温:40℃。结果:马兜铃酸A浓度在18.12～1450ng.mL-1范围内线性关系良好(r=0.9999),最低检测限为10.17ng.mL-1。结论:细辛饮片中马兜铃酸A的含量极低;本法专属性强,结果稳定,方法的回收率良好,可用于中药饮片细辛中的马兜铃酸A痕量检查。
Objective: To establish a method for the determination of traces of aristolochic acid A in Asarum decoction. Methods: The UPLC-UV method was used. The chromatographic column was Acquity UPLCTM HSS T3 column (2.1mm×50mm, 1.8μm). The mobile phase was acetonitrile (A)-0.1% formic acid (B), gradient elution (0min, 30%). A; 5 min, 60% A; 7 min, 100% A), flow rate 0.5 mL. min-1; detection wavelength 251 nm; column temperature: 40°C. Results: The linear relationship between AA concentrations in the range of 18.12-1450 ng.mL-1 was good (r=0.9999) and the detection limit was 10.17 ng.mL-1. Conclusion: The content of aristolochic acid A in Asarum Scutellariae Tablets is extremely low. This method has high specificity and stable results. The recovery rate of the method is good. It can be used for the trace determination of Aristolochic Acid A in Asarum Herbs.