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利用离子液体(Ionic liquid)的独特性质,以硅溶胶(Silica sol)为主要载体,将联吡啶钌固定到金电极上,成功制备了一种新型的电化学发光传感器。并采用循环伏安(CV)和电致化学发光(ECL)法,考察了联吡啶钌和盐酸硫必利在此修饰电极上的电化学(EC)及其发光行为。发现盐酸硫必利对固定化联吡啶钌在0.1 mol/L的磷酸盐缓冲液(PBS)介质中的弱电化学发光信号有较强的增敏作用,建立了盐酸硫必利的电化学发光新方法。在最佳实验条件下,盐酸硫必利浓度在4.0×10-7～1.0×10-4mol/L范围内与相对发光强度呈线性关系(r2=0.9962),检出限(S/N=3)为1.82×10-9mol/L。连续平行测定1.0×10-5mol/L的盐酸硫必利溶液10次,发光强度的RSD为1.6%。对样品进行回收率试验,回收率在96.7%～107.7%之间。该方法用于样品盐酸硫必利的测定,结果满意。
Utilizing the unique properties of Ionic liquid, a new type of chemiluminescence sensor was successfully prepared by immobilizing bipyridyl ruthenium on gold electrode with silica sol as the main carrier. Cyclic voltammetry (CV) and electrochemiluminescence (ECL) methods were used to investigate the electrochemical (EC) and luminescence behaviors of ruthenium biphosphate and thiopyrylhydride on the modified electrode. It was found that the thiophene hydrochloride has a strong sensitizing effect on weakly chemiluminescent signals of immobilized bipyridyl ruthenium in 0.1 mol / L phosphate buffered saline (PBS) medium, and the electrochemiluminescence method. Under the optimal experimental conditions, the concentration of thiophene hydrochloride in the range of 4.0 × 10-7 ~ 1.0 × 10-4 mol / L showed a linear relationship with the relative luminescence intensity (r2 = 0.9962). The detection limit (S / N = 3 ) Was 1.82 × 10 -9 mol / L. Successive parallel determination of 1.0 × 10-5mol / L of thiorpis hydrochloride solution 10 times, the luminous intensity RSD was 1.6%. The sample recovery test, the recovery rate between 96.7% ~ 107.7%. The method was applied to the determination of thiophene hydrochloride in samples with satisfactory results.