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目的建立里海伊蚊的实验室饲养繁殖方法,了解克拉玛依地区里海伊蚊对常用杀虫剂的抗药性水平。方法常规饲养里海伊蚊,对繁殖出的子一代采用幼蚊浸渍法,检测其对常用杀虫剂的半数致死浓度(LC50)。结果在室温(26±1)℃和相对湿度40%~60%的条件下,里海伊蚊卵孵化期为3 d,1~4龄幼虫期为7 d,从卵产下到羽化为11 d,一个卵荚孵化出110~120条幼虫。克拉玛依市敌敌畏、残杀威、溴氰菊酯、高效氯氰菊酯、双硫磷对里海伊蚊的LC50分别为0.2838、2.7752、0.0024、0.0126和0.0033 mg/L。结论克拉玛依地区里海伊蚊常用杀虫剂的LC50本底值比已经报道过的白纹伊蚊要高。为了避免交互抗性的产生,该地区里海伊蚊的防治应采取以环境治理和生物防治为主、化学防治为辅的综合治理措施,注重统筹用药和农业合理规划,定期进行抗药性监测,做到科学合理用药。
Objective To establish a laboratory breeding method of Aedes albopictus in order to understand the resistance level of Aedes aegypti to common insecticides in Karamay region. Methods The Aedes albopictus was reared in a routine manner and the larvae of the reproductive offspring were used to detect the LC50 of the commonly used insecticides. Results Under the conditions of room temperature (26 ± 1) ℃ and relative humidity of 40% ~ 60%, the egg hatching period was 3 days and the larvae of 1 ~ 4 years old were 7 days. One egg pod hatched 110 to 120 larvae. The LC50 of Karakanye, dichlorvos, propoxur, deltamethrin, beta-cypermethrin, and terephos-methyl were 0.2838, 2.7752, 0.0024, 0.0126 and 0.0033 mg / L, respectively. Conclusions LC 50 background values of commonly used insecticides in Ae. Aegypti in Karamay region are higher than those reported in Aedes albopictus. In order to avoid the emergence of cross-resistance, the prevention and treatment of Aedes albopictus in this area should take the comprehensive treatment measures that are mainly environmental treatment and biological control, supplemented by chemical prevention and control, pay attention to the integrated medication and rational planning of agriculture, regularly conduct drug resistance monitoring and do To scientific and rational drug use.