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通过对低拷贝数的转基因株京引 119的世代连续跟踪分析以及高世代材料的群体遗传分析表明 ,共转化基因bar和 cecropin B在基因组中紧密连锁并能稳定的遗传和表达。来自于转基因嘉 5 9和丙 936 3的两高世代抗除草剂材料TR5和 TR6的杂交后代分析结果显示 ,多拷贝的 bar基因以单基因表型性状传递 ,两材料中的 bar基因互不等位。TR5基因组中 ,多插入位点的 bar和 cecropin B基因在遗传转育过程中发生基因片断丢失 ,但此片段不影响转基因的表达。表明转基因事件中 ,表型性状的稳定和转基因多插入位点的纯合互不一致。
Through the generational continuous tracking analysis of low-copy-transgenic transgenic lines Jingyin 119 and the population genetic analysis of the high-generation materials, the co-transformed genes bar and cecropin B are closely linked in the genome and can be stably inherited and expressed. The analysis of progeny cross between TR5 and TR6, two high generation herbicide-resistant materials from Transgenic Jia9 and C9363, showed that multiple copies of the bar gene were transmitted as single-gene phenotypic traits, with bar genes in the two materials being unequal Bit. In the TR5 genome, multiple insertions of bar and cecropin B genes were lost during gene transfer, but this fragment did not affect transgene expression. This indicates that the stability of the phenotypic trait and the homozygosity of the multiple insertion sites of the transgene are not consistent in transgenic events.