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为了将胶原纤维光镜和透射电镜所见结合起来研究胶原纤维的细微结构,曾将一些胶原纤维的石蜡切片和冰冻切片作光镜观察,再原位环氧树脂重包埋做超薄切片电镜观察。因制片过程步骤繁多,标本受理化因素等影响,胶原纤维结构多被破坏。但在这些不成功的电镜照片中,却观察到残留下来的胶原纤维多是原纤维间横纹对位比较好的地方。由此想到原纤维间横纹对位与侧向连接牢固程度间的关
In order to collagen fiber light microscopy and transmission electron microscopy seen together to study the fine structure of collagen fibers, some collagen fibers paraffin sections and frozen sections for light microscopy, and then re-embedded in situ epoxy resin to do ultra-thin section electron microscopy Observed. Because of the many steps in the process of production, specimen acceptance factors, the structure of collagen fibers is destroyed. However, in these unsuccessful electron microscopy images, it is observed that the remaining collagen fibers are mostly the striae alignments between the fibrils. So think of the fibril horizontal stripes alignment and lateral connection between the firmness of the off