引言在过去的10年里,森林树种的试管无性繁殖已有很大的进展,已有好几篇文章列出了可用于试管繁殖的树种,如 Bajaj 等人的书都提到这个问题。因此,本文不准备描述不同树种试管无性繁殖的结果,只描述离体繁殖中所涉及的不同技术和特殊难题。植物的再生可由如下三种不同的方法获得:(1)从现有分生组织再生;(2)从不定分生组织再生;(3)通过体细胞胚再生。 INTRODUCTION Much progress has been made in the asexual propagation of test tubes in forest trees over the past 10 years. There are several articles that list the species that can be used for test tube reproduction, as mentioned by Bajaj et al. In their book. Therefore, this article is not intended to describe the results of clonal propagation in vitro of different tree species, but only describes the different techniques and particular challenges involved in in vitro propagation. Plant regeneration can be achieved by three different methods as follows: (1) regeneration from existing meristems; (2) regeneration from meristem; (3) regeneration through somatic embryos.