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目的探讨血管内皮生长因子(VEGF)蛋白含量变化与母亲妊娠高血压疾病及其胎儿宫内生长受限的相关性。方法选取2011年3月至2012年5月在我院产科进行系统性孕期监测的孕产妇及其新生儿(胎龄36~38周),妊高征组(实验组)和对照组(正常妊娠组)各纳入50对。分娩前取母体外周血,胎儿娩出后取胎盘子面和母面胎盘组织及脐血,用酶联免疫吸附法测定各标本中VEGF蛋白含量。对胎盘组织进行免疫荧光染色,对比观察两组VEGF蛋白的分布情况和荧光强度。结果妊高征组胎盘母面和子面VEGF蛋白含量均低于对照组[母面:(19.7±9.7)比(23.7±11.1)ng/ml,子面:(19.6±9.8)比(26.1±7.6)ng/ml,P<0.05],两组胎盘母面与子面VEGF蛋白含量比较差异无统计学意义(P>0.05)。妊高征组新生儿脐血VEGF蛋白含量低于对照组[(18.2±5.3)比(23.6±8.7)ng/ml,P<0.05],两组母亲产前静脉血VEGF蛋白含量差异无统计学意义[(20.5±7.9)比(21.7±8.1)ng/ml,P>0.05]。两组母亲产前静脉血VEGF蛋白含量均低于新生儿脐血,差异有统计学意义(P<0.05)。妊高征组新生儿出生体重低于对照组[(2 313±198)比(2 958±216)g,P<0.05],两组新生儿IUGR发生率差异无统计学意义(P>0.05)。结论妊娠高血压疾病母亲胎盘中VEGF蛋白含量降低与妊娠高血压的发生及其胎儿宫内生长受限有关。
Objective To investigate the relationship between changes of vascular endothelial growth factor (VEGF) protein content and maternal pregnancy-induced hypertension (BPH) and its intrauterine growth restriction. Methods From March 2011 to May 2012, pregnant women and their newborns (gestational age 36-38 weeks), pregnancy-induced hypertension group (experimental group) and control group (normal pregnancy Group) into each of 50 pairs. Maternal peripheral blood was taken before childbirth. The fetus was taken from the placenta and placenta tissues and cord blood after delivery. The VEGF protein content in each specimen was determined by enzyme-linked immunosorbent assay. Immunofluorescence staining of placental tissue was performed to compare the distribution of VEGF protein and fluorescence intensity in the two groups. Results The content of VEGF protein in the placenta of the placenta of the PIH group was lower than that of the control group [(19.7 ± 9.7) vs (23.7 ± 11.1) ng / ml, (19.6 ± 9.8) vs (26.1 ± 7.6 ) ng / ml, P <0.05]. There was no significant difference in VEGF protein content between the two groups (P> 0.05). The levels of VEGF protein in neonatal umbilical blood of PIH group were lower than those in control group [(18.2 ± 5.3) vs (23.6 ± 8.7) ng / ml, P <0.05] Significance [(20.5 ± 7.9) vs (21.7 ± 8.1) ng / ml, P> 0.05]. The content of VEGF protein in prenatal venous blood of two groups of mothers was lower than that of neonatal umbilical blood, the difference was statistically significant (P <0.05). The birth weight of neonates in PIH group was lower than that in control group [(2 313 ± 198) vs (2 958 ± 216) g, P 0.05]. There was no significant difference in the incidence of neonatal IUGR between the two groups (P 0.05) . Conclusion The decrease of VEGF protein in placenta of pregnancy-induced hypertension patients is associated with the occurrence of pregnancy-induced hypertension and the limited intrauterine growth.