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目的:建立电化学评价抗癌药物敏感试验方法。方法:以MCF-7细胞为模型,采用多壁碳纳米管修饰电极,基于MCF-7细胞质的伏安行为,研究评价抗癌药物对细胞活性的影响。结果:MCF-7细胞质在+0.738 V处出现不可逆氧化峰,并且该电化学信号可用于跟踪细胞生长曲线。电化学法检测的木犀草素、槲皮素、异槲皮素、芦丁对MCF-7细胞抑制作用均存在剂量-效应关系和时间-效应关系,并且细胞毒性效应由大到小的顺序为:木犀草素>槲皮素>异槲皮素>芦丁,同时电化学检测结果与MTT法一致。结论:所建立的电化学评价抗癌药物敏感试验方法是可靠的。
Objective: To establish an electrochemical method for evaluating anti-cancer drug sensitivity. Methods: MCF-7 cells were used as the model to modify the electrodes with multi-walled carbon nanotubes (MWNTs). Based on the voltammetric behavior of MCF-7 cytoplasm, the effects of anticancer drugs on cell viability were evaluated. Results: The irreversible oxidation peak appeared at +0.738 V in MCF-7 cytoplasm, and this electrochemical signal could be used to track the cell growth curve. Electrochemical detection of luteolin, quercetin, isoquercetin, rutin on MCF-7 cells inhibition both dose-effect relationship and time-effect relationship, and the order of cytotoxic effects descending order of : Luteolin> quercetin> isoquercetin> rutin, while the electrochemical detection results are consistent with the MTT method. Conclusion: The established electrochemical evaluation of anti-cancer drug sensitivity test method is reliable.