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５０只雌性Ｗｉｓｔａｒ大鼠，１０只行假性去卵巢术，４０只切除双侧卵巢。去卵巢大鼠再分为单纯去卵巢组、周期口服羟乙膦酸钠组、周期口服１，２５（ＯＨ）２Ｄ３组、周期序贯口服羟乙膦酸钠和１，２５（ＯＨ）２Ｄ３组。去卵巢第２６周末处死后取脾脏制备巨噬细胞，用ＭＴＴ法检测其在细菌脂多糖刺激下白细胞介素１（ＩＬ１）、白细胞介素６（ＩＬ６）和肿瘤坏死因子α（ＴＮＦα）分泌水平。结果表明，单纯去卵巢组的ＩＬ１分泌水平显著增高（Ｐ＜０．０５）；周期口服羟乙膦酸钠组在停药６周后，ＩＬ１和ＩＬ６分泌水平显著增高（Ｐ＜０．０５）；周期序贯口服羟乙膦酸钠和１，２５（ＯＨ）２Ｄ３组的ＩＬ１、ＩＬ６和ＴＮＦα分泌水平较单纯去卵巢组显著降低，近似于假性去卵巢组，亦低于单纯用羟乙膦酸钠或１，２５（ＯＨ）２Ｄ３组。提示羟乙膦酸钠和１，２５（ＯＨ）２Ｄ３联合序贯应用可使脾脏巨噬细胞ＩＬ－１、ＩＬ－６和ＴＮＦα分泌水平接近正常大鼠分泌水平，从而有可能抑制因雌激素减少所诱发的破骨细胞生成过多和活性增强，减少骨丢失。
Fifty female Wistar rats, 10 had a pseudo-ovariectomy and 40 had bilateral ovaries removed. The ovariectomized rats were divided into simple ovariectomized group, oral etidronate group and oral 1,25 (OH) 2D3 group. The rats were orally administered with etidronic acid sodium and 1,25 (OH) 2D3 . Goat ovariectomized at the end of the twelfth week after sacrifice to take the spleen preparation of macrophages, using MTT assay of bacterial lipopolysaccharide stimulation of interleukin 1 (IL 1), interleukin 6 (IL 6) and tumor necrosis factor α (TNFα) secretion level. The results showed that the level of IL-1 secretion in ovariectomized group was significantly increased (P <0.05); the level of IL-1 and IL-6 secretion in the oral etidronate group was significantly increased after 6 weeks P <0.05). The levels of IL-1, IL-6 and TNFα secreted by sequential oral etidronate and 1,25 (OH) 2D3 groups were significantly lower than those of the simple ovariectomized group Ovary group, also lower than the simple use of etidronate sodium or 1,25 (OH) 2D3 group. It is suggested that the sequential application of etidronate sodium and 1,25 (OH) 2D3 can make the levels of IL-1, IL-6 and TNFα secreted by splenic macrophages close to those of normal rats, which may inhibit the decrease of estrogen Induced osteoclastogenesis and activity increased, reducing bone loss.