应用改良式软琼脂扩散盒(ADC)进行人癌克隆形成试验,检测26例6种手术切除肿瘤标本,培养成功率为65.4%,铺板率在0.003～0.814%之间,接种细胞与克隆形成之间有明显的线性关系(P<0.05)。采用ADC技术筛选铂兰化合物,初步试验显示该药能抑制集落形成,结果与动物实验一致。 Human colorectal cancer cell clone formation assay was performed with an improved soft agar diffusion kit (ADC). Twenty-six resected tumor specimens were detected. The successful rate of culture was 65.4% and the plating rate was between 0.003% and 0.814% There was a significant linear relationship between (P <0.05). Screening platinum compounds using ADC technology, preliminary tests showed that the drug can inhibit the formation of colonies, the results and animal experiments.