论文部分内容阅读
目的研究血清和脑脊液中髓鞘碱性蛋白(MBP)及S-100B蛋白在急性脊髓损伤后以及应用川芎嗪治疗后的变化及其临床意义。方法用Allens打击法制作犬的T13节段急性脊髓损伤模型,于蛛网膜下腔留置一枚硬脊膜管,并将一端穿出皮外封闭,留做采脑脊液用。实验动物随机分三组:A组(n=6)为对照组,B组(n=6)脊髓损伤组,C组(n=6)川芎嗪治疗组。三组分别伤后2、4、6、8、10、24、48、72、96h采静脉血4mL、脑脊液1mL离心,-20℃保存待测。ELISA法检测血清和脑脊液中的MBP和S-100B蛋白的含量。结果急性脊髓损伤后,脊髓损伤组和川芎嗪治疗组中血清和脑脊液中MBP及S-100B含量均显著升高且其水平呈动态变化,与对照组比较差异有显著性意义。川芎嗪治疗组升高水平低于脊髓损伤组,并在72h时差异有显著性意义(MBP)。结论脊髓损伤后血清和脑脊液中MBP、S-100B呈动态变化,川芎嗪对急性脊髓损伤有治疗作用,MBP和S-100B可作为急性脊髓损伤的标志物并值得对其进行进一步研究。
Objective To investigate the changes and clinical significance of MBP and S-100B protein in serum and cerebrospinal fluid after acute spinal cord injury and after treatment with Ligustrazine. Methods A dog model of acute spinal cord injury in T13 was made using the Allens method. A dural tube was placed in the subarachnoid space, and one end was pierced out of the skin and left as a cerebrospinal fluid. The experimental animals were randomly divided into three groups: group A (n=6) was the control group, group B (n=6) spinal cord injury group, and group C (n=6) ligustrazine treatment group. Three groups of blood were collected at 2, 4, 6, 8, 10, 24, 48, 72, and 96 hours after injury. 4 mL of venous blood and 1 mL of cerebrospinal fluid were centrifuged and stored at -20°C. The levels of MBP and S-100B proteins in serum and cerebrospinal fluid were detected by ELISA. Results After acute spinal cord injury, the levels of MBP and S-100B in serum and cerebrospinal fluid were significantly increased in the spinal cord injury group and the tetramethylpyrazine treatment group, and their levels were dynamically changed. There was significant difference compared with the control group. The elevated level of ligustrazine treatment group was lower than that of spinal cord injury group, and there was a significant difference (MBP) at 72 hours. Conclusion The changes of MBP and S-100B in serum and cerebrospinal fluid after spinal cord injury are dynamic. Ligustrazine has a therapeutic effect on acute spinal cord injury. MBP and S-100B can be used as markers of acute spinal cord injury and deserve further study.