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目的探讨使用三氯乙烯(TCE)染毒对豚鼠肝功能和肝细胞凋亡基因(BAX、BAD、Bc1-2)表达的影响。方法将24只豚鼠随机分为3组,采用豚鼠最大值法(GPMT),设立TCE实验组、阴性对照组、阳性对照组,用皮内注射的方式分别注射TCE、橄榄油、2,4-二硝基氯苯(DNCB),实验结束后观察动物皮肤改变,应用自动生化分析仪检测动物肝功能指标,用荧光定量PCR检测肝细胞凋亡基因表达水平。结果 TCE实验组和阳性对照组动物出现明显皮肤损害。TCE实验组动物血清中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、乳酸脱氢酶(LDH)活力明显高于阴性对照组(P<0.05或P<0.01)。肝细胞BAX、BAD的mRNA表达水平比阴性对照组显著升高,Bc1-2表达水平下降(P<0.05或P<0.01)。结论三氯乙烯可诱导豚鼠产生明显的皮肤变态反应,引起实验动物肝功能指标改变和肝细胞凋亡基因表达水平明显改变。
Objective To investigate the effects of TCE on the liver function and the expression of hepatocyte apoptosis genes (BAX, BAD, Bc1-2) in guinea pigs. Methods Twenty-four guinea pigs were randomly divided into three groups. TCE group, negative control group and positive control group were established by guinea pig maximum method (GPMT). TCE, olive oil, 2,4- Dinitrochlorobenzene (DNCB) was used to observe the skin changes of animals. The liver function indexes of animals were detected by automatic biochemical analyzer, and the expression of apoptosis genes was detected by real-time PCR. Results TCE experimental group and positive control group of animals showed significant skin lesions. The serum levels of ALT, AST and LDH in the TCE experimental group were significantly higher than those in the negative control group (P <0.05 or P <0.01). The mRNA expression of BAX and BAD in hepatocytes was significantly higher than that in the negative control group, while the expression of Bcl-2 was decreased (P <0.05 or P <0.01). Conclusion Trichlorethylene can induce significant skin allergic reaction in guinea pigs, causing changes of liver function index and expression of hepatocyte apoptosis gene in experimental animals.