白喉菌苗的效力可通过在豚鼠中诱导抗体来检测,抗体水平用毒素中和试验(TNT)检测。虽然这是一种广泛使用的可靠方法,但具有耗时、费力以及费用大等缺点。基于以上原因,作者改用有高度敏感性及重复性的ELISA测试用疫苗免疫的豚鼠血清中白喉抗毒素(抗-DT)水平来评价疫苗效力。 The potency of Diphtheria bacterin can be detected by inducing antibodies in guinea pigs and antibody levels detected by the toxin neutralization assay (TNT). Although this is a widely used and reliable method, it has the disadvantages of time consuming, laborious and expensive. For these reasons, the authors evaluated the vaccine efficacy using diphtheria anti-toxin (anti-DT) in guinea pig serum immunized with a highly sensitive and reproducible ELISA test vaccine.