AFLP and SCAR Markers Associated with Peel Color in Eggplant (Solanum melongena)

来源 :Agricultural Sciences in China | 被引量 : 0次 | 上传用户:tzxue
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
Peel color is an important breeding objective for eggplant.Dark purple and purplish red are the most common colors in commercial eggplant cultivars.A co-dominant amplified fragment length polymorphism (AFLP) marker which was associated with the peel color (each in coupling phase to dark purple and purplish red) was found in studying the genetic diversity in 58 eggplant accessions (contained cultivars and wild relatives).The maker bands were sequenced and converted to SCAR marker,this polymorphism in sequence was from an inserted/deleted (indels) mutation.And DNA from 136 eggplant materials (inbred lines,F1,and wild relatives) were amplified with the designed SCAR primers as template,high correlation between the SCAR marker and peel color (dark purple and purplish red) was found.Then,bulked line analysis (BLA) combined with AFLP was further used to identify polymorphic fragments,and another six AFLP markers were tested and verified to be associated with peel color,which demonstrated that BLA was an useful method for identifying molecular markers of interested traits.In conclusion,these markers will facilitate the MAS (maker-assisted selection) of eggplant breeding for peel color. Peel color is an important breeding objective for eggplant. Dark purple and purplish red are the most common colors in commercial eggplant cultivars. A co-dominant amplified fragment length polymorphism (AFLP) marker which was associated with the peel color (each in coupling phase to dark purple and purplish red) was found in studying the genetic diversity in 58 eggplant accessions (contained cultivars and wild relatives). The maker bands were sequenced and converted to SCAR marker, this polymorphism in sequence was from an inserted / deleted (indels) mutation . DNA DNA from 136 eggplant materials (inbred lines, F1, and wild relatives) were amplified with the designed SCAR primers as template, high correlation between the SCAR marker and peel color (dark purple and purplish red) was found. Chen, bulked line analysis (BLA) combined with AFLP was further used to identify polymorphic fragments, and another six AFLP markers were tested and verified to be associated with peel color, which demonstrated tha t BLA was an useful method for identifying molecular markers of interested traits. In conclusion, these markers will facilitate the MAS (maker-assisted selection) of eggplant breeding for peel color.
其他文献
本文从中学图书馆阅读推广的意义、中学图书馆阅读推广发展的必然性、中学图书馆有效开展课外阅读指导策略三方面入手,旨在激发学生自主阅读的兴趣,提高学生自主阅读的能力,