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目的 :探讨IL 6、IL 1β、TNFα在人胎大脑神经细胞体外发育过程中对原癌基因c fos、c jun的表达调控规律。 方法 :应用人胎大脑神经细胞无血清原代培养模型 ,通过DNA RNA斑点杂交 ,采用计算机图像分析系统测定杂交点的平均灰度值。结果 :c fos、c jun的表达均在IL 6、IL 1β、TNFα作用后 15～ 30min开始上调 ,1h达高峰 ,而后下降。 结论 :这些细胞因子均在转录水平上促进神经细胞c fos、c jun的表达 ,为分析其生物学效应的分子机制奠定基础
AIM: To investigate the regulation of c fos and c jun expression of IL 6, IL 1 β and TNFα in human fetal brain neurons in vitro. Methods: The primary culture model of human fetal brain neuron was established. The average gray value of hybridization point was determined by computerized image analysis system by dot blot of DNA and RNA. Results: The expressions of c fos and c jun began to rise at 15-30 minutes after IL 6, IL 1β and TNFα treatment, reached the peak at 1 hour and then decreased. Conclusion: All these cytokines promote the expression of c fos and c jun at the transcriptional level, which lays a foundation for analyzing the molecular mechanism of their biological effects