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1．取材：用上腹部无病变的尸体，①横断十二指肠上端、横断并结扎肝十二指肠韧带；②横断十二指肠下端、从胰头下平面横断并结扎肠系膜上动、静脉；③贴近脾门切断并结扎脾动、静脉；①从腹腔动脉发出以上和肠系膜上动脉发出以下切断腹主动脉；⑤沿十二指肠及胰周围游离此二结构。2．插管：切开十二指肠壁和腹主动脉后壁，分别从十二指肠大乳头至胰管、腹腔动脉开口、肠系膜上动脉开口三处插入平头大号注射针头并结扎固定。3．灌注；先从胰管灌入20％过氯乙烯（溶于乙酸乙酯中，加黄色颜料）10～15ml，只要被膜下能见黄色细管即止；再从腹腔动脉和肠系膜上动脉分别注入红色20％过氯乙烯40～50ml及5～10ml，见胰腺膨胀。只要见被膜下出现红色细管即止。一小时后、再补充灌注一次，用量为1／4左右。4留置：将已灌注标本于室内放置12小时，使灌注液中乙酸乙醇挥发，过氯乙烯固化、标本中液体溢出。5腐蚀：置标本于浓盐酸中（50％到70％）7天后，取出轻轻用清水冲洗、修洁后即可用于观测。
1. Drawn: the upper abdomen without lesions of the body, ① transected the upper end of the duodenum, transverse and ligation of the hepatoduodenal ligament; ② transection of the lower end of the duodenum, transverse transection of the head of the pancreas and ligation of mesenteric upper and lower venous; ③ close to the spleen and cut off and splenic artery ligation; ① issued from the celiac artery and the superior mesenteric artery issued the following cut off the abdominal aorta; ⑤ free from the duodenum and pancreas around the two structures. 2. Intubation: The duodenal wall and the posterior wall of the abdominal aorta were dissected from the large duodenal papilla to the pancreatic duct, the celiac artery, and the superior mesenteric artery respectively. 3. Perfusion; first perfusion from the pancreatic duct 20% perchlorethylene (dissolved in ethyl acetate, yellow pigment) 10 ~ 15ml, as long as the capsule can see yellow tubules; then from the celiac artery and superior mesenteric artery were injected Red 20% perchlorethylene 40 ~ 50ml and 5 ~ 10ml, see the pancreatic expansion. As long as the thin red tube appears under the envelope. An hour later, then add a perfusion, the dosage is about 1/4. 4 indwelling: the perfusion specimens were placed indoors for 12 hours, perfusion solution of acetic acid in ethanol, perchlorovinyl solidification, the sample liquid overflow. 5 Corrosion: Set the sample in concentrated hydrochloric acid (50% to 70%) for 7 days, remove gently rinse with water, after repair can be used for observation.