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本文首次报道感染抗氯喹‘NS’系小鼠血清对氯喹抗疟药效具有拮抗作用。将抗氯喹‘NS’系(抗性为20～30倍)和正常伯氏疟原虫(Plasmodium berghei)株分别接种两组昆明株小鼠,另设一组无原虫感染的小鼠作对照。于接种后第5d自三组小鼠采血清,采血前检查前两组原虫感染率,分别为13和22％,按无菌操作,自心脏抽血,将血液置于37℃使其充分凝固,析出的血清经1500～2000rpm离心5min后置于4℃保存备用。用感染‘NS’系小鼠血清(SMNS)、感染正
In this paper, for the first time, it is reported that the serum of mice infected with anti-chloroquine NS has an antagonistic effect on the efficacy of chloroquine antimalarial. Two strains of Kunming strain were inoculated respectively with chloroquine-resistant NS strain (20-30 fold resistant) and normal Plasmodium berghei strain. Another group of mice without protozoal infection were used as controls. At 5 days after inoculation, the mice of three groups were harvested for sera. The infection rates of the two groups of protozoa before the blood sampling were 13 and 22% respectively. Blood was drawn from the heart by aseptic technique and the blood was allowed to fully coagulate at 37 ° C The precipitated serum was centrifuged at 1500-2000rpm for 5min and then stored at 4 ℃ for later use. Infected with ’NS’ mouse serum (SMNS)