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2008年8—9月对胶东半岛葡萄主产区15个葡萄园的病毒病发生及危害情况进行了田间调查,采集了4个酿酒葡萄品种共计44份葡萄样品,采用双抗体夹心酶联免疫吸附法进行病毒血清学检测。从样品中分别检出葡萄斑点病毒(GFKV)、葡萄卷叶病毒1(GLRaV-1)和葡萄卷叶病毒3(GLRaV-3),检出率分别为6.82%、2.27%和40.91%。蛇龙珠样品中3种病毒均被检出,总检出率达60.71%;赤霞珠样品中检出了葡萄卷叶病毒3和葡萄斑点病毒,总检出率为36.46%。蓬莱地区葡萄样品中3种病毒均被检出,检出率达55.56%,龙口地区病毒检出率也达到了52.17%。
From August to September in 2008, field investigation was conducted on the occurrence and damage of viruses in 15 vineyards in the main grape producing areas of Jiaodong Peninsula. A total of 44 grape samples from 4 grapevine varieties were collected and detected by sandwich enzyme-linked immunosorbent assay Law for viral serology. Gram-spot virus (GFKV), grapevine leaf curl virus 1 (GLRaV-1) and grapevine leaf curl virus 3 (GLRaV-3) were detected from the samples with the detection rates of 6.82%, 2.27% and 40.91%, respectively. Three kinds of viruses were detected in the snake dragon and pearl samples, with a total detection rate of 60.71%. Grapevine leaf curl virus 3 and grapevine spot virus were detected in Cabernet Sauvignon samples with a total detection rate of 36.46%. Penglai grape samples were detected in three kinds of viruses, the detection rate of 55.56%, Longkou area virus detection rate reached 52.17%.