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对Rowell氏还原银染色法稍加改良,可以在体外培养的鸡胚大脑神经细胞及背根神经节细胞获得清晰的染色效果,尤其便于观察纤细的突起。该方法的特点是显影液中含硝酸银。控制显影过程的速度可大大地改善此方法的重复性。本文还讨论了其他一些问题并介绍了重新染色的方法。
Rowell’s reduction of silver staining slightly improved, in vitro culture of chicken embryonic brain cells and dorsal root ganglion cells to obtain a clear staining results, in particular, to facilitate the observation of slender protrusions. The method is characterized in that the developer contains silver nitrate. Control of the development process can greatly improve the repeatability of this method. This article also discusses other issues and describes ways to re-stain.