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目的观察维拉帕米对兔脊髓缺血再灌注神经损伤的影响。方法选用健康兔30只,随机分为3组,A组为假手术组(只穿线不结扎),B组为缺血再灌注组(腹主动脉缺血30 min后,再灌注48 h),C组为维拉帕米组(0.2 mg/kg维拉帕米静脉注射后,腹主动脉缺血30 min,再灌注48 h)。对3组动物行神经功能评价并通过病理组织学观察神经元细胞的变化,以此评价维拉帕米对兔脊髓缺血再灌注神经损伤的保护作用。结果 A组神经功能评价、神经元细胞的病理组织学观察均正常。B组神经功能出现明显障碍,神经元细胞形态异常。C组亦出现神经功能障碍,神经元细胞形态异常,但均较B组轻。结论应用维拉帕米可减轻因脊髓缺血再灌注造成的神经损伤。
Objective To observe the effect of verapamil on the spinal cord ischemia-reperfusion injury in rabbits. Methods Thirty healthy rabbits were randomly divided into three groups. Group A was sham operation group (only threading did not ligate), group B was ischemia reperfusion group (reperfusion 48 hours after ischemia of abdominal aorta) Group C was treated with verapamil (0.2 mg / kg verapamil intravenously after 30 min ischemia and 48 h reperfusion). The neurological function of three groups of animals was evaluated and the changes of neurons were observed by histopathology to evaluate the protective effect of verapamil on nerve injury induced by spinal cord ischemia-reperfusion in rabbits. Results A group of neurological evaluation, neuronal cell histopathology were normal. B group showed obvious neurological dysfunction, abnormal neuronal cell morphology. C group also showed neurological dysfunction, abnormal neuronal cell morphology, but were lighter than the B group. Conclusion The application of verapamil can reduce the nerve injury caused by spinal cord ischemia-reperfusion.