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目的观察糖皮质激素对骨髓基质细胞增殖及分化的作用,探讨糖皮质激素导致继发性骨质疏松的病理学机制。方法骨髓基质细胞以随机数字表法随机分为对照组和实验组。以1×10-7mol/L地塞米松加入髓基质细胞培养物中,测定增殖的骨髓基质细胞数及培养液中骨钙素含量。通过苏丹III脂肪细胞染色计数观察地塞米松作用时间对脂肪细胞分化的影响。结果实验组及培养液中骨钙素含量(5.2±1.4)ng/mg蛋白质,明显低于对照组(12.5±1.5)ng/mg蛋白质(t=12.61,P<0.001);骨髓基质细胞数实验组(3.73±0.43)×105个细胞/孔,明显低于对照组(5.76±0.69)×105个细胞/孔(t=7.45,P<0.001);脂肪细胞的数量随地塞米松作用时间延长而增多。结论糖皮质激素抑制骨髓基质细胞增殖及向成骨细胞分化,促进其向脂肪细胞分化,这可能与糖皮质激素引起继发性骨质疏松时,骨量减少,髓内脂肪组织增多有关。
Objective To observe the effect of glucocorticoids on the proliferation and differentiation of bone marrow stromal cells and to explore the pathological mechanism of glucocorticoid-induced secondary osteoporosis. Methods Bone marrow stromal cells were randomly divided into control group and experimental group by random number table method. 1 × 10-7mol / L dexamethasone was added to the culture of marrow stromal cells, and the number of proliferating bone marrow stromal cells and the content of osteocalcin in the culture fluid were measured. The effect of dexamethasone duration on adipocyte differentiation was observed by Sudan III adipocyte staining. Results Compared with control group (12.5 ± 1.5) ng / mg protein (t = 12.61, P <0.001), osteocalcin (5.2 ± 1.4) ng / mg protein in experimental group and culture medium was significantly lower than that in control group (3.73 ± 0.43) × 105 cells / well was significantly lower than that of the control group (5.76 ± 0.69) × 105 cells / well (t = 7.45, P <0.001). The number of adipocytes increased with the dexamethasone increase. Conclusion Glucocorticoid inhibits the proliferation of bone marrow stromal cells and differentiates into osteoblasts and promotes their differentiation into adipocytes. This may be related to the increase of bone mass and intramedullary adipose tissue induced by glucocorticoid induced secondary osteoporosis.